First data of ITS1-genotyping of the Black Sea trematodes Cainocreadium and Helicometra (Trematoda: Opecoelidae)

Main Article Content

A. V. Katokhin

http://orcid.org/0000-0003-4537-456X

https://elibrary.ru/author_items.asp?id=88361

Yu. M. Kornyychuk

http://orcid.org/0000-0003-3095-6436

https://elibrary.ru/author_items.asp?id=696720

Abstract

Data of genetic analysis of the Black Sea trematodes of Cainocreadium genus and Helicometra fasciata were obtained for the first time. The nucleotide sequences of ITS1 rRNA gene cluster of Cainocreadium flesi from Platichthys flesus (GenBank entries MG980645, MG980646) and Cainocreadium sp. from Gaidropsarus mediterraneus (MG980643, MG980644, MK248037, MK248038) off Crimean Black Sea coast were found to be identical. Nevertheless, they have not been synonymized because of morphological differences known between these hostal morphs. Sequences of Cainocreadium from the Black Sea fish turned out to differ, by 4 positions, from similar sequences of a closely related Mediterranean congener, C. labracis (cercaria). Five insertions-deletions and 38 nucleotide sequences distinguish the ITS1 sequences of the Black Sea trematodes, C. flesi and Cainocreadium sp., from ITS1 sequences of another Mediterranean congener, C. dentecis. The ITS1 sequences of the Black Sea and Mediterranean Helicometra fasciata samples also differ: 5 nucleotide changes and 11 insertions-deletions were identified. Supplementary data associated with this article can be found in online version at https://doi.org/10.21072/mbj.2018.03.4.04.

Article Details

Keywords
ITS rDNA, Opecoelidae, Cainocreadium, Helicometra, Black Sea
Section
Scientific communications

References

1. Гаевская А. В., Корнийчук Ю. М. Паразитические организмы как составляющая экосистем черноморского побережья Крыма // Современное состояние биоразнообразия прибрежных вод Крыма (черноморский сектор) / под ред. В. Н. Еремеева, А. В. Гаевской ; НАН Украины, Институт биологии южных морей. Севастополь : ЭКОСИ-Гидрофизика, 2003. С. 425–490. [Gaevskaya A. V., Kornyychuk Yu. M. Paraziticheskie organizmy kak sostavlyayushchaya ekosistem chernomorskogo poberezh’ya Kryma. Sovremennoe sostoyanie bioraznoobraziya pribrezhnykh vod Kryma / V. N. Eremeev, A. V. Gaevskaya (Eds); NAN Ukrainy, Institut biologii yuzhnykh morei. Sevastopol: EKOSI-Gidrofizika, 2003, pp. 425–490. (in Russ.)].

2. Корнийчук Ю. М. Трематоды рода Cainocreadium (Opecoelidae) в Черном и Средиземном морях // Паразитология в XXI веке – проблемы, методы, решения : материалы IV Всерос. съезда Паразитологического общества при Российской академии наук, Санкт-Петербург, 20–25 окт. 2008 г. Санкт-Петербург : Лема, 2008. Т. 2. С. 77–80. [Kornyychuk Yu. M. Trematody roda Cainocreadium (Opecoelidae) v Chernom i Sredizemnom moryakh. Parazitologiya v XXI veke – problemy, metody, resheniya: materialy IV Vseros. s’ezda Parazitologicheskogo obshchestva pri Rossiiskoi akademii nauk, Saint Petersburg, 20–25 Oct., 2008. Saint Petersburg: Lema, 2008, vol. 2, pp. 77–80. (in Russ.)].

3. Корнийчук Ю. М., Гаевская А. В. Cainocreadium flesi sp. n. (Trematoda, Opecoelidae) – новый вид трематод черноморских рыб // Вестник зоологии. 2000. Т. 34, вып. 6. С. 89–91. [Korniichuk Yu. M., Gaevskaya A. V. Cainocreadium flesi sp. nov. (Trematoda: Opecoelidae) – a new trematode species from the Black Sea fishes. Vestnik zoologii, 2000, vol. 34, iss. 6, pp. 89–91. (in Russ.)].

4. Bioinformatics Tools: Multiple Sequence Alignment: Clustal Omega [Program]. URL: https://www.ebi.ac.uk/Tools/msa/clustalo/ (accessed 20.10.2018).

5. Blend C. K., Dronen N. O. A review of the genus Helicometra Odhner, 1902 (Digenea: Opecoelidae: Plagioporinae) with a key to species including Helicometra overstreeti n. sp. from the cusk-eel Luciobrotula corethromycter Cohen, 1964 (Ophidiiformes: Ophidiidae) from the Gulf of Mexico. Marine Biodiversity, 2015, vol. 45, iss. 2, pp. 183–270. https://doi.org/10.1007/s12526-014-0250-3.

6. Born-Torrijos A., Kostadinova A., Raga J. A., Holzer A. S. Molecular and morphological identification of larval opecoelids (Digenea: Opecoelidae) parasitising prosobranch snails in a Western Mediterranean lagoon. Parasitology International, 2012, vol. 61, iss. 3, pp. 450–460. https://doi.org/10.1016/j.parint.2012.03.002.

7. Jousson O., Bartoli P. Molecules, morphology and morphometrics of Cainocreadium labracis and Cainocreadium dentecis n. sp. (Digenea: Opecoelidae) parasitic in marine fishes. International Journal for Parasitology, 2001, vol. 31, iss. 7, pp. 706–714. https://doi.org/10.1016/S0020-7519(01)00180-1.

8. Jousson O., Bartoli P., Pawlowski J. Molecular identification of developmental stages in Opecoelidae (Digenea). International Journal for Parasitology, 1999, vol. 29, iss. 1, pp. 1853–1858. https://doi.org/10.1016/S0020-7519(99)00124-1.

9. Kiyan V. S., Bulashev A. K., Katokhin A. V. Opisthorchis felineus and Metorchis bilis metacercariae in cyprinid fish Leuciscus idus in Nura-Sarysu River, Kazakhstan. Korean Journal of Parasitology, 2018, vol. 56, iss. 3, pp. 267–274. https://doi.org/10.3347/kjp.2018.56.3.267.